Module 2C: Delivery of Opsins

In this presentation, Mario Penzo will describe the various strategies commonly employed for the delivery of bacterial opsins to the nervous system. Specifically, Penzo will:

• Describe different opsin delivery methods, such as virus injection, electroporation, and use of transgenic mice.
• Identify, for a particular delivery strategy (adeno-associated virus vectors), the importance of the various serotypes available.

After watching this presentation, you should be able to understand the pros and cons of the different opsin delivery methods.

Visit the Community forum for all eight modules to share your insights and best practices, ask questions, and engage with other training series’ participants.

Speaker

Mario A. Penzo, PhD

Mario A. Penzo is the chief of the unit on the neurobiology of affective memory of NIH National Institute of Mental Health (NIMH). His group’s research aims to understand the neuronal mechanisms underlying the formation and regulation of affective memories. To do so, they combine various technical approaches, which include behavioral assays, electrophysiology, neuroanatomy, and optogenetics. Penzo obtained his PhD. at the Albert Einstein College of Medicine and completed his postdoctoral fellowship at Cold Spring Harbor Laboratory.